In successive partitioning of 50/50 acetone and water extract with n-hexane, chloroform, ethyl acetate and butanol, what solvent system can be used for TLC and column chromatographic separation of ethyl acetate fraction?
Debu Kumar Bhattacharjya
In the case of TLC you can try Dichloromethane( 100%---) and Methanol ( 2.5%---). And for column chromatography, you can try n-hexane and ethyl acetate (1:1---).
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Cyril Ogbuanu
Thanks, Is the DCM (100%)to be combined methanol (2.5%).
I will try it.
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