What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Quantification of GC-MS data, issue with Masshunter, Any ideas?

Hi, I am using the Masshunter software to quantify my GC-MS data. So, did the integration using MassHunter. but the data (area) are weird. I have a lot of peaks in one chromatogram, therefore,...

09 November 2020 2,181 5 View

How to apply cyclic loading to a discrete model of a masonry wall in 3DEC software?

It is possible to apply a monotonic load to a URM wall in 3DEC code but I am wondering is it possible to apply a cyclic load there like FEM softwares?

10 February 2020 3,237 3 View

Can pET expression vectors get used for sequencing and storage?

I was wondering if we could skip one step in cloning process from sequencing vector to expression vector? Meaning that start cloning the gene directly into expression vector such as pET 28b and do...

04 February 2020 9,742 2 View

Do you recommend ligation free cloning system?

I plan to clone some terpene synthase genes into a sequencing vector and later do the heterologous expression using an expression vector. I was wondering if anyone has experience with free...

28 January 2020 7,751 3 View

Why R studio deletes some rows from the heatmap/ doesn't show all genes?

I am using Rstudio to create heatmap for some expression data; My file includes 38 genes, therefore, 38 rows and 20 columns as samples. The output heatmap shows only 19 of my genes!!! Any idea how...

10 December 2019 7,530 3 View

Problem with trimAL installation?

Is anyone can help in how to install trimAL on windows 10? I am gonna use the software for trimming of my aligned sequences. It seems the software does not get installed in my system! Any idea...

15 September 2019 6,068 3 View

What will be the programming language of future Computational Codes? Good old FORTRAN evolves or will be removed from heavy duty computational world?

I know there are great reliable libraries on Fortran for Computational PDE/ODE, Computational Statistics and Numerical Linear Algebra. Also almost all the legacy software packages of Civil...

30 August 2018 5,424 4 View

Do I need to design a specific antibody for purification of my small recombinant peptides?

I am fusing small peptides around 10 to 30 a.a into the C-terminus of a larger protein. The question is if I need to design specific antibodies against those small epitopes? I actually have two...

10 May 2018 1,091 1 View

How pET29 expresses the HIS-tag? Is it possible that a truncated recobminant protein or a his-tag is expressed after IPTG induction?

I have cloned my gene of interest into pET29c, and sequencing verified it too. I used a c-terminus his-tag in my insert. However, when i do induction (1mM IPTG, Over night at 28C), I see a band...

11 January 2018 2,466 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View