Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Measuring viscosity and density under high pressure?

I need the density and viscosity of a molecular fluid under relatively high pressures, I expect something around 500 MPa. This kind of measurement is way outside my field, and I can find only very...

19 October 2020 2,812 3 View

What formula to get the number of masks needed to wear to prevent 1 infection?

Hi everyone ! I have a challenging time to crack the code in the following analysis...

27 July 2020 3,915 1 View

"Internal solution magnitude limit was exceeded". How to solve this question in Ansys Workbench?

I am modelling a foundation connection to a wind barrier. The connection is 4 prestressed bolts with an anchorage plate in the bottom, it is all cealed in a clump of concrete. When I change the...

29 October 2019 4,944 2 View

Does anyone know an easy and simple method of detecting trichloroacetic acid (TCA) and/or trifluoroacetic acid (TFA) in solution?

I am investigating electrokinetic migration of these acids across a supported liquid membrane (SLM), and need to determine their presence (if any) in the aqueous acceptor phase. I am thinking...

17 October 2018 9,594 4 View

How to add response bias into a repeated measures mixed anova model?

Hi everyone! I have a 2 repeated measures that was asked at 3 moments in time (within-subject factor). We doubled the measure to control for response bias. So the measure (likert scale) probed...

02 April 2018 2,389 3 View

Oscillating behaviour in ITC data - any explanations?

Hi, When doing ITC in our lab we have recently observed an osculating behaviour of the raw ITC data. We have tested different systems and the behaviour is the same (however, most visible with...

06 March 2018 1,117 6 View

SaOs-2 cells and lipofectamine-2000?

20 April 2016 1,781 3 View

BiFC and ImageJ threshold?

Hi, I'm currently working on the dimerization of two solute carriers. For that I'm using bi molecular fluorescence complementation. I've transfected 293FT cells attached to poly-D-lysine coated...

21 February 2016 4,440 3 View

How can I calculate functional hill numbers?

I would like to calculate the functional diversity measures proposed by Chiu & Chao (2014). The paper gives formulas for the calculation of functional hill numbers, mean functional diversity...

08 June 2015 9,731 7 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

PMSM Inter Turn Short Circuit fault, Dataset?

Hi, I'm looking for a dataset of a PMSM with an inter turn short circuit fault. Does anyone know a dataset which is available?

21 February 2021 5,663 2 View