I intend to make artifical urine following the protocol of Chutipongtanate and Thongboonkerd (Systematic comparisons of artificial urine formulas for in vitro cellular study - 2010). What should I expect the final solution to look like?
Will it has any colour? Any cloudiness? Any specific odour?
Hi,
this depends a lot on the application you have in mind. From you topics I assume that you want to test a medical device. There are standards available (like EN 1616), which test the stability of a certain material and they might be sufficient. For long term application or applications with pharmaceuticals you should also consider that materials become encrusted and that components that are not considered in the standard like proteins may interact with the material/drugs.
We used an artificial urine which is colourless and without odour or cloudiness. But we used just an aqueous solution with the different ions dissolved on it. This allow us to maintain the ionic strength and the pHs values as the ones usually present in urine. However, maybe it is too simple for your purposes, and you should add other organic compounds such as citrate, creatinine, uric acid, urea...
I attached you a link where our protocol for preparing AU is described, in case you need it.
Best regards
http://link.springer.com/article/10.1007%2Fs00240-012-0473-3
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