As per OECD guideline, the exposure time to determine the acute toxicity should be 96 hrs. I want to know the reason why it is continuous 96 hr exposure. Is there any specific reason to take this time duration for exposure?
I think that a serious discussion is taking place about the rigor with which one works on experimental models such as zebrafish. I could not give an answer about times of exposure without knowing the protocol in detail and the objectives of the work ... 96 hours from fertilization the individual has stopped being a larva.
I send here a recent work on how to face the rigor of work with zebrafish. The same concept of median lethal dose, LD50 is debatable since for example thalidomide has teratogenic effects in humans but rats do not show teratogenesis.
Regards!!
María Beatriz Espinosa thanks for your interest ..
The protocol we are going to follow will be same as OECD 203 and the objective of work is to determine the dose of drug to further find out the efficacy of the same.
Regards....
Hi,
Zebrafish (Danio rerio) has been a prominent model vertebrate in a variety of biological.the large-scale screening for toxicity of several hundreds of chemicals at a time of the larval and adult zebrafish minimizes costs through low quantities of dosing .these genes can be assessed for disruption after chemical exposure. At the end of the exposure period, acute toxicity is determined...
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