I want conducting SARA analysis but i am unable to determine what dia column should i use and how much height od silica bed is required to effectively separate maltenes into 3 components.
I am calculating viscosity of my oil in water emulsion using a rotational viscometer with a disk shaped spindle. The fluid is less viscous upon observation. However when i measured the viscositu,...
04 May 2019 9,867 4 View
I have an oil in water emulsion of which i want to find interfacial tension using a pendant drop tensiometer. what is the correct procedure for the same?
04 May 2019 500 2 View
I have an oil in water emulsion in which more oil is dispersed in less amount of water, the oil is viscous in nature. the emulsion seems stable when kept in tubes while it the same seems unstable...
02 March 2019 3,072 3 View
If an oil in water emulsion made by mixing oil in water using surfactant separates after sometime, then can it be mixed again to form the emulsion? Kindly provide references (if any) along with...
02 March 2019 6,837 1 View
I am working on oil in water emulsion. i wanted to know that if i make a 250 ml emulsion of 60% oil and 40% water containing 1000 ppm surfactant and remove 50 ml from it for stability analysis....
09 October 2018 409 4 View
09 October 2018 4,142 1 View
I want the detailed procedure for SARA analysis, solvents required, apparatus and also detailed procedure on how to perform column chromatography with multiple solvents.
08 September 2018 7,230 4 View
I am working on crude oil and want to conduct SARA analysis which involves sequential elution with multiple solvent systems (4 as per ASTM procedure) i wany to know how to change solvent system in...
08 September 2018 8,976 3 View
I want to add polymeric additive to my sample but i want to add it in ppm level concentrations so i don't know how to draw few microlitres of viscous liquid. i tried with a micropippete but some...
07 August 2018 7,600 0 View
what is the mechanism by which the viscosity of heavy crude is reduced? what type of polymers are used as viscosity reducers? are they commercially available?
06 July 2018 2,190 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
… with my present female lover … she and I spend anywhere from two hours to six hours in caressing, touching, cuddling, hugging, lip kissing, deep kissing and intimate conversation before,...
11 August 2024 4,521 0 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View