Hello the smartest scientists on the earth. I am evaluating the antibacterial effect of 25nm and 50nm cerium oxide nanoparticles and I need to evaluate the production of ROS of my antibacterial nano-systems. I used a flurescence kit to test the ROS levels. The ROS I tested is singlet oxygen and hydroxyl radicals. In my result, I found that 25nm CeO2 NPs produce more singlet oxygen than 50nm NPs and less hydroxyl radicals than 50nm NPs. However in the literature I read it seems that smaller size the more hydroxyl radicals will be produced.
I also have my nanoparticles to have TEM analysis, we do not have equipments in our school so I sent samples to other institutions for TEM analysis. But due to the nanoparticles are commercial product, I cannot identify the morphology of the nanoparticles. The 50nm nanoparticles seems to be triangle and octahedral shape and 25nm seems to be mixed.
I am still learning as a beginner of nanoparticles and I hope someone can tell me what shape of cerium oxide nanoparticle will produce more hydroxyl radicals or singlet oxygen? Or in a mixed condition there will be less ROS produced?
Chloe Park
CeO2 nanoparticles are capable of performing the functions of the enzyme catalase and destroying hydrogen peroxide. CeO2 nanoparticles are capable of inactivating hydroxyl radicals. These are examples of the size effect of CeO2 nanoparticles. The size effect properties always increase with decreasing nanoparticle size.
@Yuri Mirgorod Thank you for your answer! I understand that the size affect the ros greatly. I am worried because there is only 25nm or even smaller size difference between them
Chloe Park
Compare the specific surface area m2/cm3 the difference will be much greater. The specific surface area determines the size effect.
What ROS can be produced from 25nm and 50nm cerium oxide nanoparticles?
There are discrepancies regarding the toxicity of cerium oxide, such as studies that report 5 cerium(III) oxides. Cerium oxide has applications in catalysis, modulator of oxidative stress in living organisms, in biomedicine, among others. The effect of five cerium oxide (Ce) nanoparticles with different characteristics has been reported in a model organism: for example: the microalgae Pseudokirchneriella subcapitata.
How to plot Dielectric parameters Vs frequency, received from LCR meter outputs (Capecitance and Dissipation Factor)?
Steps to follow: 1) collect capacitance and dissipation factor data for a series of frequencies
2) Use a spreadsheet (such as Microsoft Excel or Google Sheets) or data analysis software (such as Python with Matplotlib or R).
How can I determine the precise cell parameters of a phase in powder diffraction?
Determining the precise cellular parameters of a phase in powder diffraction is a fundamental process in the characterization of crystalline materials. Here are the steps and methods commonly used to make this determination:
1) Sample preparation
2) Obtaining diffraction standard
3) Identification of diffraction peaks
4) Determination of interplanar distances
5) Determination of crystal structure
6) Calculation of unit cell parameters
7) Adjustment and refinement. Rietveld refinement through the use of FullProf software. For simple tuning you can use Python
8) Analysis
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