What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

21 February 2021 9,133 2 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

Antibody fragments after Dha (Dehydroascorbic acid) re-oxidation?

Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...

31 January 2021 9,495 2 View

How to analyze dendritic spines from tissue (Prelimbic cortex)?

Hi there, I am trying to measure dendritic spines from mouse fixed tissue, specifically, I am looking at the prefrontal cortex. I injected the PrL with a lentivirus to knock-down my protein of...

27 January 2021 9,251 2 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Do you think NCC and 3 year service at army base must be compulsory to make the students loyal and discipline to the nation?

Decipline and loyalty for nation is the need of today.

09 February 2021 6,928 3 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

Would you like to participate in our study by completing the questionnaire?

04 February 2021 7,661 1 View

How is it possible to rank variables using Likert scale?

Hello Everyone, I have a questionnaire of a 3-point Likert scale for an overall evaluation for a service, and several detailed attributes evaluation of that service. For example: Overall...

31 January 2021 9,612 8 View

What is the best and affordable method to make and distribute a survey?

What is the best and affordable method for higher education student to build and distribute a survey in the United States of America with consideration of the following points: There are no...

23 January 2021 9,342 2 View

What are your opinions on Small Software as a Service (Saas) Sustainability?

Hello, I am conducting a research survey for my dissertation to compare academicians' and practitioners' opinions on Small Software as a Service Sustainability). Would you kindly participate in...

19 January 2021 6,591 2 View

How to calculate random forest manually?

17 January 2021 301 2 View

Is there and standard of Chemical price when writing a SCI paper?

Hello I writing a SCI paper and want to make a table for chemical price comparison. However, the chemical price varies from country and company, amount and also dates. Is there any reference...

12 January 2021 5,600 1 View