What protocols do you use for fungi cryopreservation with glycerol?

Isaias Perez Ramirez @Isaias-Perez-Ramirez

01 August 2023 1 2K Report

Let me give you some background, we have isolated fungus from cocoa tissue. Our next objective is to cryopreserve the samples with glycerol. We´re looking for a simple, but effective method for conservating our samples. Do you know of any?

I share with you a photo of our isolated fungus.

Povilas Kavaliauskas

we typically harvest the spores and suspend them in YPD broth with 50% glycerol. we store them at -80.

The technique works for most human fungal pathogens, both molds and yeast.

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