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Questions related from Sundar Khadka
What percentage of IL-17 positive cells are expected to be positive in Th17-polarized cells in flow cytometry? Day-1: Coating anti-CD3 and anti-CD28 Day 0: Seeding of purified CD4 cells +...
16 May 2024 7,031 0 View
Hello all; We tried to make antibiotic cocktail (0.1% each) in the tap water (200 microliter)for oral gavage to SJL/J mice. Antibiotics cocktail: 0.1% Ampicillin (=0.01gm)+ 0.1% Metronidazole +...
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Hello all; Antibiotic administration in the drinking water of mice: How to improve drinking habits of mice? We tried antibiotic cocktail in the drinking water of SJL/J mice. Antibiotics...
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Hello all; We are going to quantify the bacterial DNA of individual bacteria in feces at the GENUS level by PCR. Although we tried to find the primer sets from published papers, we were not able...
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Hello all; We are going to quantify bacterial load of individual bacteria at GENUS level from DNA extracted from feces, and ileal contents specimens. Could you help us how can we find primers sets...
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What percentage of IL-17 positive cells are expected to be positive in Th17-polarized cells in flow cytometry? Day-1: Coating anti-CD3 and anti-CD28 Day 0: Seeding of purified CD4 cells + Cytokine...
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