What percentage of Alamar blue is used for the proliferation assay?
Alamar Blue may be used at a concentration ranging from 0.5 - 50 μM, preferably at a concentration of about 5 μM. You should add Alamar Blue in an amount equal to 10% of the culture volume in the well.
What number of cells to be seeded in 96 well plate for proliferation assay?
Optimal seeding density is 1 x 10^4 cells/ml. But it may vary between different cell lines. You could use a seeding density ranging from 5000 to 40,000 cells/ml depending on your cells.