I am checking the genetic stability of my isolated stem cells and I am using a genetic analyzer to detect any MSI. I am using five microsatellite markers; two mononucleotide repeats (BAT25,BAT26) and three dinucleotide repeats (D5S346,D2S123, and D17S250).
I need to know the exact procedures, from extracting DNA from cells to getting the PCR product that can be sequenced.
Thank you in advance.
Dear Nermeen,
I hope this link will be useful for you
http://jmd.amjpathol.org/article/S1525-1578(10)60164-X/pdf
All the best
Thank you so much, the paper has been so informative, but I need to know more about the technical procedures and steps.
I think microsatellite PCR products are hard to sequence & give errors, either u clone the fragment of if u want sizing do fluorescent based genotyping, one more thing your DNA should be of good quality
Dear Nermeen,
this Promega's manual contains detailed procedures related to MSI
http://www.promega.com/~/media/files/resources/protocols/technical%20manuals/0/msi%20analysis%20system1%202%20protocol.pdf
All the best
- Badges
- Science topic