What more reliable technique to measure alpha linolenic acid and carnitine in the seminal plasma of stallions?

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How do you deal with your enironmental chemistry data?

Hi everybody! I am PhD in analytical chemistry and I make part of a multidisciplinary team working on the development of a web based solution focusing mainly on treatment and management of...

12 March 2020 5,814 5 View

Does it exist any open repositories of user stories?

For a Machine Learning-based project, I'm looking for repositories containing user stories (and acceptance criteria). This can be issue management systems (e.g., stories in Jira) or plain...

19 December 2018 4,442 7 View

Does anyone have experience with BDNF Emax® Immunoassay System of PROMEGA?

We're in doubt about the dilution and acidification of the serum human samples. It's really necessary to proceed the 1:4 dilution of each test sample and preparing 1:2 serial dilutions per sample...

20 December 2015 2,016 1 View

What is the best indicator of product profitability?

The literature on the subject is very poor. As a result, many companies use inappropriate methods, which are based on fixed cost sharing.

27 November 2014 2,717 10 View

How to improve pregnancy rates in dairy cows using the TAI using P4 / E2?

Over the past five years with field data using different protocols TAI in dairy herds realized that ovsynch and pre-synch walks very well with protocols similar to those sold in our country...

19 November 2014 3,854 5 View

Does anyone know the best procedure to avoid pigments with the minimal loss of auxin in the leaf extracts?

Thinking in the lifetime of LC/MSMS the leaf pigments (mainly chlorophyll) are not welcome. However, some procedures that we used (DSC-18 or activated charcoal) reduced the Signal/Noise ratio to...

15 October 2014 5,018 6 View

Does anyone have a good supplier and catalog number for ATRA (all-trans retinoic acid) to differentiate human iTregs?

ATRA is often cited in papers, but I'm not sure which format to buy as there are a lot of different ones listed in Sigma.

28 May 2014 8,996 1 View

Does anyone know of a site/blog/forum where people post their experience with particular antibody titration clones and fluors?

Titrating antibodies is time consuming and expensive. I know that concentration/conditions varies greatly based on the particular assay. However, does anyone know of a site/blog/forum where...

20 April 2014 8,396 0 View

What is the consensus on human Th1 differentiation?

I'm trying to differentiate purified (>95%) human naive CD4+ T cells in vitro. This topic has been addressed several times in various settings, but I wanted to get thoughts on specific “tricks”...

07 April 2014 6,079 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View