i m doing gcms analysis of plant extracts (Bruker, 436 GC). i derivatized the samples nd final vol is 300 microlitre. is this vol sufficient for gcms analysis in a 2 ml vial so that injector can get it
More the concentrated is your sample less it requires for the analysis. Although all it depends upon concentration of the sample even then minimum 75% vial of capacity 2ml should be filled for the best results.
In addition to the vial fill, concentration, piercing etc. precautions which are very contributing in analysis, check the limit of detection of your machine and be well above it, x10, if possible. Go through the manual and see the recommended operating procedures along with other indications on fruitful analysis. The manual can also be had online.
thank you all. we found an option where injector needle could be adjusted for low volume samples.
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