Different levels of tested IBA failed to induce rooting in cold hardy ornamental bamboo. Provide the reference or suggestions.
Dear Faiq,
I suggest a factorial experiment set up with reducing the strength of major elements in your medium, different IBA levels and sucrose levels. Even reducing sucrose (if using 3 %, try 2 and 1%) and the strength of the major elements (if using full strength, try half and 1/4th) with 3 mg/L IBA will solve the problem.
Here is an interesting reference:
Ramanayaka et al (2006) In vitro shoot proliferation and enhancement of rooting for the large-scale propagation of yellow bamboo (Bambusa vulgaris ‘Striata’) Scientia Horticulturae. 110, (109-113). Last part of the abstract says - "Rooting was enhanced when the relatively difficult-to-root in vitro shoots from adult field culms were pretreated with 0.5 mg L−1 TDZ for two to three subcultures before placing in the root induction medium. Continuously illuminated shoots pretreated with TDZ for three subcultures showed 100% rooting compared to 83% rooting of shoots that were exposed to a 12 h photoperiod. These findings have been applied in the large-scale propagation of this species."
So, why not try continuous illumination and if still problematic, subculture in TDZ supplemented media before going for continuous illumination?
Good luck.
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