What might be an explanation for the presence of qPCR amplified viral RNA genomic product in mock wells?

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Nicolas Tremblay

Are you transfecting plasmids for you virus production?

Gabriel R Anderson

Yes, viral plasmids were transfected into HEK-293 cells

If you have transfected a transfer plasmid (WPRE) in your mock wells, you could have carry over DNA (DNA that was not removed from your RNA isolation) in your qPCR reaction that results in a false positive.