i am interested in the methods because I am currently about to conduct entomological (Aedes) survey of public schools installed with insecticide-treated screens.
Standard methods for assessing the resistance of wild mosquito populations to insecticides are WHO topical tests and tube tests (impregnated paper).
World Health Organization, 2002. Supplies for monitoring insecticide resistance in disease vectors, procedures and conditions, WHO/CDS/CPE/PVC/2001.2, 16 p.
Insecticide resistance of field populations of Aedes aegypti can be assessed using WHO (1975) insecticide susceptibility test kits & standard procedures for larvae (WHO 1981a) & adult (WHO 1981b). Adults are exposed to impregnated papers with the WHO discriminating concentrations of test insecticides. A susceptible strain (i.e Bora bora) can be used as a positive control & for resistance ratio determination in comparison with the test field strain.
Monitoring and managing insecticide resistance in Aedes mosquito populations: Interim guidance for entomologists. WHO 2016. you can refer to this.
Using WHO Insecticide resistance treated papers with the guideline of WHO (2016) or you can as well use CDC bottle bioassay.
Meanwhile if am to open talks on what you intend doing, Aedes species majorly are day biters mostly biting outdoors. Except the screen is for the whole school and not inside the hostels or classroom but i will be happy to see the outcome. Gracias
We are using the WHO test kits for mosquitoes susceptibility test and following the Guide lines of the susceptibility test, you can visit the WHO web site and you can also for more to see this link for more details https://r.search.yahoo.com/_ylt=AwrEzee22aRbm_IAqW5XNyoA;_ylu=X3oDMTByOHZyb21tBGNvbG8DYmYxBHBvcwMxBHZ0aWQDBHNlYwNzcg--/RV=2/RE=1537559094/RO=10/RU=https%3a%2f%2fwww.ncbi.nlm.nih.gov%2fpmc%2farticles%2fPMC3121691%2f/RK=2/RS=Pk2GRhskqIMxKxYWiG3sswtV.dU-
you can use protocol provided by WHO for testing resistance.These files may help you.
https://www.cdc.gov/malaria/resources/pdf/fsp/ir_manual/ir_cdc_bioassay_en.pdf
http://www.who.int/malaria/publications/atoz/WHO-insecticide-resistance-test-procedures-2016-presentation-en.pdf
Using WHO susceptibility test kit, CDC bottle bioassay can be used as complementary. Please do search for the protocol online.
I conduct mosquito studies in the Philippines. Where is the nearest source of WHO cones for insecticide-treated screen bioassay?
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