I have already tried adapting established methods for other animals with no success. I am using a polyclonal antibody (Sigma).
This paper will for your case
Analysis of the Drosophila compound eye with light and electron microscopy.
Mishra M, Knust E.
Methods Mol Biol. 2013;935:161-82. doi: 10.1007/978-1-62703-080-9_11.
Hi Giulia,
probably you want to try the line OK371 reported in the paper: http://dx.doi.org/10.1016/j.modgep.2005.07.006
the line is available in bloomington with the stock number 26160
http://flystocks.bio.indiana.edu/Reports/26160.html
Probably you want to cross it with GPF or other reporter and either visualize it in vivo or after IHC.
Good luck!
Abud
Really really useful, thanks a lot!
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