I want to use an anion exchange chromatography to remove impurities from a mAb production broth. What would be the maximum ingoing titer of the mAb in the loading step of the AEX?
With this I don't mean the binding capacity of the resin but ingoing mAb titer in the broth.
Thank you in advance!
There is no general answer for this.
It depends on the characteristics of your antibody, the other impurities, like DNA and HCP and their concentrations, process-related impurities (i.e., stuff you added during the upstream process), your buffer matrix, pH, ionic strength, temperature, ...
You have to test and optimize it for your specific conditions.
Should have thought of this earlier: determine the dynamic binding capacity of your resin with your choice of conditions.
See, for instance, here for some instructions:
https://www.gelifesciences.com/en/us/news-center/how-to-determine-dynamic-binding-capacity-10001
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