I fabricated elecrtochemical biosenser for glucose detection and i showed the response time but i can not understand the reviewer comment of (A comment on the time constant of the sensor response would also be helpful for comparison). from the i-t test i showed the respons time but i coiuld not understand what maening time constant of the sensor response?
second Q:
one of the reviewer asked Why the Fe-O band shifted from Fe3O4 to magnetite- prussian blue (PB)?
I characterize Fe3O4 and composite Fe3O4 PB nanoparticles by IR and the result of Fe3o4 showed absorption peak of Fe-O bonds at 573 cm-1 and for IR of composite Fe3O4-PB it showed absorption peak of Fe-O bonds at 596 cm-1. according to the literature both band attributed to the Fe-O bonds.
so he asked why there is defferent in the bans of Fe-O in Fe3O4 and Fe-O band in composite Fe3O4-PB?
Also he asked why there is no negative transmittance in IR spectra?
Iam accaching the FTIR spectra of pure Fe3O4 nanoparticles (a) and magnetite PB nano-composites (b).
A. If possible, attach the i-t curve.
B. The peak shift in higher wavenumber implies that the composite is formed.
C. FTIR data has some issues as it shouldn't go to the negative transmittance.
A simple time constant sometimes used is the time to reach 90% or 95% of the final signal from the start of a step change in glucose concentration. This typically would be a few seconds at most.
Also he asked why there is no negative transmittance in IR spectra?
The background to infra-red spectroscopy.
In IR spectra, a percentage transmittance of 100 would mean that all of that frequency passed straight through the compound without any being absorbed. In practice, that never happens - there is always some small loss, giving a transmittance of perhaps 95% as the best you can achieve in your IR results.
About the biosensor 'time constant' please check my answer at the related question: https://www.researchgate.net/post/Can_anyone_help_me_understand_a_comment_on_the_time_constant_of_the_sensor_response
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