How can I detect IL-1B by Western blot?

Hello Everyone, I know IL-1B is a soluble protein. So how to extract it from the supernatant? Please advice. Thanks!

05 January 2021 7,349 5 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Are there any EEG facilities for hire?

I need to run a quick EEG experiment. I don't want to spend time and money to set my own lab. I wonder if there exist EEG labs that can run an experiment for me for a fee?

10 September 2020 5,492 3 View

How do you report undetermined Ct values?

I have knocked down a gene of interest using siRNA and am using qRT-PCR to confirm reduced expression. All samples treated with non-specific siRNA have Ct values around 35, but all samples...

29 July 2020 8,198 3 View

Use of spherical silver?

Where is silver in spherical shape used other than for solar cell electrodes? Most of the silver in electronics seems to be flake. Is this statement accurate?

24 May 2020 7,604 3 View

Insect Population Counting and Studying Traps - recommend a good book or source for info on?

Hi, I'm looking for an entomologist that can recommend a good book on the trapping of insects for study. Thanks

30 January 2020 2,710 2 View

IHC: Monocyte vs Macrophage?

How would you distinguish peripheral monocytes from tissue macrophages with IHC? Specifically, I am interested in looking at rodent brain tissue. Any help is appreciated! Thanks, Dan

07 October 2019 2,683 3 View

Where can I find data on urban land cover in the UK from the 1960s?

I'm modelling insect populations over time (1968 - present) in the UK at over 500 sites. I have detected a decline in sites that are mainly suburban (based on 500m radii using the CEH Land Cover...

02 September 2019 535 3 View

Would (groundwater) dissolved noble gas concentration change after isolation from gas exchange with air?

Dear scientists of noble gas and hydrology, Would equilibrium and/or excess concentrations of noble gases change after isolation from gas exchange with atmosphere? (Non-atmospheric noble gas was...

20 February 2019 255 5 View

Is there a way to control for categorical groups to calculate partial correlations for various measurements?

I have a sample size of 30, representing three different groups (one intervention and two controls). I would like to see if there is an association between my dependent variable and 4 self-report...

18 February 2019 4,619 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View