I need an protocol for treating wastewater sample in Airlift Bioreactor using natural adsorbent materials like banana peel, algae and some other materials?
If I have framed your question correctly, a number of 'bioreactor' materials could be deployed to treat wastewater. One such example is tree barks which could successfully remove ammonium from wastewater. The finer the particle size greater the efficiency of removal. Bark can be treated with slacked lime to enable phosphorus absorption as well. What is critical is retention time, which varies according to the type of effluent and bark material used. The reaction is primarily carried out by chemical process (ion exchange) than biological.
Similar bark materials, depending on their ability to supply dissolved carbon can also be used to strip nitrate from wastewater through denitrification process which is a biological reaction.
I think some more stable natural materials may be better, such as nut shells, chitosan, which are physical strong to be used as packing material and porous to be used for adsorption of metals and emulsified oils.
My main concert is why to use bioreactor if the process you intend to develop is based on physical/chemical mechanisms. There is plenty of literature on airlift bioreactor
For an updated review check: Guieysse B, Quijano G, Muñoz R (2012). Bioreactors – Design | Airlift Bioreactors. In: Comprehensive Biotechnology, Second Edition. Edited by: Murray Moo-Young, volume 2, pp. 199–212. Elsevier. ISBN: 978-0-444-53352-4.
If you want to remove both organic and inorganic contaminants from waste water, then you need a both absorbant materials like fruit peels or biomaterials like chitosan etc and also bacteria which can perform the functions like organic carbon oxidation, nitrification, denitrification etc...but, if you try to do these in a single reactor system, you may end up with the complete bacterial biofilm formation on the surface of adsorbant materials. so, first you may have to treat the waste water with adsorbants followed by the organics removal in bioreactor.
Lot of work has been done on the recent past on airlift bioractor. An example of this can be found in the attached reprint.
Article Biodegradation of Tributyl Phosphate, an organosphate triest...
it depends on the adsorbant materials you are planning to use...if you use materials like chitosan or pollen grains or any material with good physical strength for inorganics removal, it may not change the composition of waste water. but, if u use materials like fruit peels which have less strength, they may slightly change the quality of water in terms of total organic content (toc/cod) atleast after few cycles of their use. but if u intend to use bioreactor in the second stage for organics removal, that will take care of toc....