I am considering to purchase the EuroClone SafeGrow 188 Direct Heat CO2 Incubator for growing of sensitive pluripotent stem cells. Have you good experience from this incubator?
We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...
21 January 2021 2,969 1 View
Hi, does anyone know why TRAP staining faids in FFPE rat femur sections? My protocol: After deparaffination and rehydration i put the slides in Acetat Buffer for 20min, then they go to the...
14 December 2020 3,112 4 View
Anyone with experience in growing JAWS II cells could give me an idea of its doubling time? I intend to use these cells for transfection protocols. Any tips to help with growing and transfecting...
01 December 2020 855 3 View
I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...
08 September 2020 5,436 3 View
We undergone so much problems with NVivo (activation, compatibility PC vs MAC) and it is so much expensive.
07 July 2020 8,222 10 View
Hello, Excluding contamination and probe degradation, can anyone suggest a cause of late amplification in the NTC of TaqMan qPCR ? I considered the possibility of primer dimers, but since they...
21 March 2020 8,904 3 View
I am experiencing low 260/230 values after using Qiagen's DNeasy PowerSoil Pro Kit. I have been getting values anywhere from around 0.11 to 1.10. After speaking with a Qiagen rep, she recommended...
03 March 2020 4,203 7 View
Dears, I used 4% PFA to stain in vitro cultured cells for protein staining (Cristal Violet). No I would like to stain lipid droplets in the cell with oil-red solution. I thought I will NEED...
06 February 2020 2,010 1 View
Looking for a place to submit an article related to assessment and evaluation in physical education that was rejected clearly due to being qualitative in nature.
06 February 2020 3,229 2 View
I am using the rabbit anti-mouse IgG-CFL 488 sc-362259 from Santa Cruz and because some problems we performed a test using the secondary antibody without the primary and we realized this antibody...
15 January 2020 2,947 1 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...
01 March 2021 1,907 3 View
I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...
01 March 2021 5,475 2 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View