Cyanobacteria (blue-green algae) are single celled photosynthetic organisms that utilize Chlorophyll-a and phycocyanin which gives it the bluish tint that it is known for. There are some properties of Cyanobacteria that make it similar to that of Chloroplast, and it can thus be assumed that chloroplast and cyanobacteria stem from a common
ancestor and share many similar qualities.
Cannot use 540 for cynobacteria. OD 730 nm is mostly used for cyanobacterial suspensions. Simultaneously measuring at 665 nm will give the amount of chlorophyll in the sample. So it is better to use both of them to get the cell density as well as the chlorophyll content at 730 and 665 nm respectively.
I have a similar problem. I measured an unknown strain of cyanobacteria growing in suspension at 730 nm. What exactly is measured at 730 nm, though?
And can anyone explain to me please, why OD 730 drops when the cyanobacteria is put under limitation and increases again after sometime? Is there some type of downregulation of the protein that is measured at 730 nm?