Amina,

You need to harvest the brain as soon as the animal dies. Waiting for 24 hours post PTZ seizures may not be fesible for all animals if the mortality rate is different for differnt animals. The protein degradation will commence immediately after death and your biochemical read out will not be accurate.

Thank you for your answer Dr. Puranam

In case the animal does not die within 24 hrs, shall I fix a cut off time of 24 hrs? what about the biochemical read out in that case?

Amina,

As a start, yes fixing the cut off time at 24 hrs seems reasonable. The most important question is what are you trying to answer and detect? Expression of some proteins with seizure? If so, data on some proteins like BDNF is avaialble in literature. You can start with the 24 hr cut off and based on your results, modify your protocol to address the questions you have set out to answer.

I recommend you refer to Stanley Wiegard’s paper in Experimental Neurology 148, 398-410, 1998.

If you do not have accesses to the paper, send me your email and I will send you a PDF.

Good luck with your experiments

Thanks a lot for your assistance Dr. Puranam

If you send me the PDF of the paper you mentioned, I'll be grateful. My e-mail is [email protected].

Best regards

Amina

which parameters you will evaluate is important time of removing brain. For example if you want to evaluate apoptosis you will remove brain as possible as quicliy 24 hours after your study. I advise you check your parameters optimal expressing time in literature.

Best Regars

Ahmet Sevki

Thank you Dr. Ahmet for your answer

Thank you Dr. Sadangi. I'll definitely consider your advice.