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Questions related from Badrul Haidar
In our lab we are working on different bacterial communities. Accidentally we have found a bacteria (as contamination) which showing positive activity on some plate assay (secondary metabolites)....
08 August 2018 9,555 6 View
I am trying to purify the bacterial protein (3kd size) by C18 column (NUCLEODUR® C18 ec, 250 mm, 4.6 mm, 5 µm, 110 A◦) on HPLC. Firstly, I am running semi preparative run and collect the...
11 November 2017 9,487 6 View
By qualitative assay, we have found some bacteria showing positive to utilize nitrogen in Nfb media. Now we are trying to quantify nitrogen fixation by these endophytic bacteria. So if anyone...
08 August 2016 2,177 2 View
We are screening antimicrobial activity of endophytic bacteria.Some of it have shown antimicrobial activity against Staphylococcus aureus by agar overlay assay. We also tried from supernatant but...
04 April 2016 4,526 10 View
I have isolated fungal extracellular metabolites with ethyl acetate . Now I am trying to separate unknown metabolites by hplc. What kind of solvent system I should choose? . I got good...
12 December 2015 6,853 5 View
I extracted fungal extract (extra cellular ) with ethyl acetate . I want to do some bioassays for this.Now I want to check cytotoxic effect of this extract. which assay will be effective for crude...
10 October 2015 6,932 8 View
We have grown some plant endophytic bacteria in TSB medium. Now we are trying to grow them in liquid medium (TSA) for genomic DNA isolation ,some are growing and some are not ,what can be the reason?
02 February 2015 10,006 11 View
I am trying to amplify unknown 5'end of a gene in jute plant by inverse PCR. Everytime I set the PCR and got bands for it. I sent it for sequencing but I found that it is from non target region...
08 August 2014 7,119 9 View
