I work on the MIP to detect the testosterone in water. Colloidal crystals of silica particles were fabricated on the glass slide. Then, I used acrylic acid as a monomer, EGDMA as a cross linker and AIBN as an initiator. My problem is when I made the incubation to detect the hormone, the concentration of testosterone before and after the incubation is the same ( I used 30 mg of MIP in 15 ml of different concentration solution of hormone). This mean there is no detection, or my MIPs not work. Also, I got the same results for NIP. 

Notes: 

1-  I used the hydrofluoric acid (HF) for removal of silica particles after polymerization and acetic acid for removal of testosterone from the MIP. 

2- Also, my MIPs is a thin film, not particles

Does anyone have the same problem when worked on the MIP or anyone can advise me, please?

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