What is the transfer condition for handmade 1.5mm 15% gel to transfer histone H4?

I AM USING 20% METHANOL IN TRIS GLYCINE , 0.2 MICRON NITROCELLULOSE MEMBRANE FOR 100 V HR . the ponceau staining looks weaker compared to precast gel 10-12% with the transfer using sds and...

02 March 2018 327 3 View

Does molten magensium react with nickel curcible?

08 September 2014 7,456 4 View

Can we store Annexin V FITC stained cells and use after a week?

Can we stain the cells with annexin v fitc and fix with pfa and then store? If yes, can anyone suggest the optimized protocol for flow cytometry?

03 April 2014 8,449 6 View

Does the primary Ab and secondary Ab from different companies matter?

I am using cell signalling primary antibody and secondary antibody from sigma and substrate is NBT/BCIP. primary Ab dilution 1:1000 (3hrs rocking) sec Ab 1:10000 (45min rocking) primary ab...

02 March 2014 1,594 6 View

How do you add output styles in Endnote web?

I want to add the XXX journal output style in endnote web. In my endnote web only 21 output style are available, e.g. numbered, Ris, science, etc.

02 March 2014 6,054 12 View

What might be the reason for ghost bands in western blot?

What might be the reason for ghost bands in western blot? 1. I have used 0.5% skim milk overnight blocking 2. Primary antibody dilution 1:1000 3hrs rocking (3xwash PBST/ PBS) RT 3.Secondary...

01 February 2014 6,593 6 View

Why are bands are not visible in PVDF membrane after being stained with ponceau?

I have transferred gel in PVDF membrane and stained with ponceau but I couldn't see any bands on the membrane, instead, the whole PVDF membrane is turned into pink color and the color is not going...

11 December 2013 2,417 3 View

What is the difference between scrapping and trypsinising cells for extracting protein?

Is there any difference in protein content or alteration in phosphorylation of protein by using scrapping method and trypsin method for doing western blot?

10 November 2013 354 9 View

Why protein from SDS PAGE is not transferred onto nitrocellulose membrane?

While I'm transferring the protein from SDS gel to Nitrocellulose membrane only marker is getting transferred but not protein. I used 100v for 1hr to transfer. Can we reuse a transfer buffer? Is...

08 September 2013 8,118 21 View

Can we reuse a primary antibody which is diluted skimmed milk powder? If yes, can anyone suggest storage conditions?

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01 February 2013 6,862 9 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View