Asiya Parvin

16 Questions 19 Answers 0 Followers

Questions related from Asiya Parvin

What is the transfer condition for handmade 1.5mm 15% gel to transfer histone H4?

I AM USING 20% METHANOL IN TRIS GLYCINE , 0.2 MICRON NITROCELLULOSE MEMBRANE FOR 100 V HR . the ponceau staining looks weaker compared to precast gel 10-12% with the transfer using sds and...

03 March 2018 354 3 View

Does molten magensium react with nickel curcible?

Does melting magnesium at 650 degrees in nickel crucible (bought from sigmal adlrich) react with nickel crucible?

09 September 2014 7,480 4 View

Can we store Annexin V FITC stained cells and use after a week?

Can we stain the cells with annexin v fitc and fix with pfa and then store? If yes, can anyone suggest the optimized protocol for flow cytometry?

04 April 2014 8,478 6 View

Does the primary Ab and secondary Ab from different companies matter?

I am using cell signalling primary antibody and secondary antibody from sigma and substrate is NBT/BCIP. primary Ab dilution 1:1000 (3hrs rocking) sec Ab 1:10000 (45min rocking) primary ab...

03 March 2014 1,631 6 View

How do you add output styles in Endnote web?

I want to add the XXX journal output style in endnote web. In my endnote web only 21 output style are available, e.g. numbered, Ris, science, etc.

03 March 2014 6,123 12 View

What might be the reason for ghost bands in western blot?

What might be the reason for ghost bands in western blot? 1. I have used 0.5% skim milk overnight blocking 2. Primary antibody dilution 1:1000 3hrs rocking (3xwash PBST/ PBS) RT 3.Secondary...

02 February 2014 6,624 6 View

Why are bands are not visible in PVDF membrane after being stained with ponceau?

I have transferred gel in PVDF membrane and stained with ponceau but I couldn't see any bands on the membrane, instead, the whole PVDF membrane is turned into pink color and the color is not going...

12 December 2013 2,442 3 View

What is the difference between scrapping and trypsinising cells for extracting protein?

Is there any difference in protein content or alteration in phosphorylation of protein by using scrapping method and trypsin method for doing western blot?

11 November 2013 400 9 View

Why protein from SDS PAGE is not transferred onto nitrocellulose membrane?

While I'm transferring the protein from SDS gel to Nitrocellulose membrane only marker is getting transferred but not protein. I used 100v for 1hr to transfer. Can we reuse a transfer buffer? Is...

09 September 2013 8,169 21 View

Can we reuse a primary antibody which is diluted skimmed milk powder? If yes, can anyone suggest storage conditions?

02 February 2013 6,891 9 View

Can I use secondary antimouse IgM for primary Phospho-Akt (Ser473) (587F11) Mouse mAb #4051?

Can i use secondary antimouse IgM for primary Phospho-Akt (Ser473) (587F11) Mouse mAb #405v1 for western blot?

07 July 2012 8,602 13 View

How to do pI3 kinase assay by using PI3 Kinase Activity/Inhibitor Assay Kit (millipore)?

I want to do PI3 Kinase Activity assay by using millipore PI3 Kinase Activity/Inhibitor ELISA kit in that protocol they didn't mention the details about the sample volume in the the...

07 July 2012 2,177 1 View

How to calculate 100 microMolar solution of COCl2. 6H2O?

M.Wt of COCl2.6H20 is 237.93 For stock sol 25mM 5.95mg dissloved in 1ml ddH20 if i take 4 microliter from the stock and make up into 1ml can i get 100microMolar solution? If i take 4 from the...

06 June 2012 2,478 8 View

What is the step by step protocol for DCF assay?

I searched in many article i want to measure Ros during cocl2 treatment after 24 hr after drug pre treatment in adherent cells by using plate reader and also in con focal but i am confused...

06 June 2012 8,620 4 View

Can I use HRP linked secondary antibody for western blot? Are there any extra procedures I should follow?

04 April 2012 5,422 3 View

Can I use HRP linked secondary antibody for western blot? Are there any extra procedure that I should follow?

Read 3 answers by scientists to the question asked by Asiya Parvin on Apr 4, 2012

04 April 2012 8,742 2 View