Healthy and Major Depressive Dissorder Connectome data?

Hello all, I am currently starting my master's thesis and I would like to use real data instead of simulating some parameters. For that, I need to find some healthy and MDD connectome, in order...

02 March 2019 1,466 1 View

How should we proceed with the diagnosis of rodents that transmit diseases such as hantavirus?

I have seen works in which authors do not describe how they have determined the specimens. Systematics seems to be a discipline totally abandoned in the local environment of my Country. What...

23 January 2019 2,404 1 View

What kit for intracellular NAD determination do you recommend?

I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...

08 August 2018 1,608 1 View

CD4+ T cell lines expressing and not expressing CD38?

17 September 2017 5,509 2 View

Which classes should i create for my R package?

Dear all, I have developed an R package here https://github.com/ibarraespinosa/vein This package depends on sp but it works with simpler classes. Mi intention is to create classes but i would like...

21 April 2017 2,572 2 View

No title

When expressed in these two ways depending if it is heme content or pigment, heme iron is expressed in g hematine/ 100g muscle, g myoglobin/ 100g meat, g heme iron/ 100 g muscle (are this...

17 February 2017 7,594 1 View

What is the difference between Myc and cMyc tag proteins?

We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...

12 September 2016 419 6 View

What is the best LPS to induce inflammation in mice by ip injection?

LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...

18 May 2016 6,426 5 View

Can the maximal expression in an experiment be used as reference delta cT to calculate deltaDelta cT (Instead of the basal expression)?

03 November 2014 427 4 View

How much change in Ct is acceptable for a housekeeping gene?

We are analyzing memory CD4 T cell responses (cytokines, co-stimulatory molecules and chemokine receptors) before and after TCR-mediated stimulation. GAPDH gave nice parallel efficiencies with the...

24 September 2013 4,262 12 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View