what is the screening strategy for isolation of alginate producing bacteria from soil?
The usual methods for EPS producers should work, followed by using alginate lyase to screen those which produce alginate. The two attached reprints should give you a start.
am hydrolysing corncob powder with 1% sulphric acid (autoclaved) then the solid and liquid portion were separated by filtration process. in liquid portion i calculated the total sugars but am not...
07 August 2016 1,425 2 View
kindly help me to calculate percentage acid hydrolysis of corncobs with sulphuric acid (1%), am getting 10gL Xylose during process
06 July 2016 10,071 3 View
Why RID in HPLC is ideal for the quantitative estimation of sugars ? why not PDA ?
05 June 2015 6,504 4 View
i am looking for spectrophotometry estimation of 5HMF ?
02 March 2015 4,682 1 View
I am looking for phylogenetic analysis is aldoketo reductase family protein, while searching the sequence related to the same in NCBI. Aldo-keto reductase and aldoketo reductase giving different...
01 February 2015 4,396 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
What is the behavior of anaerobic bacteria in deep salty groundwater with high level of chlorine? Is the nitrate level deceasing and is denitrification controlled by excess chlorine? Thanks
13 July 2024 1,581 4 View