How to calculate total sugars in hydrolysed corncob solid matter( after hydrollysis with sulphuric acid)?

am hydrolysing corncob powder with 1% sulphric acid (autoclaved) then the solid and liquid portion were separated by filtration process. in liquid portion i calculated the total sugars but am not...

07 August 2016 1,246 2 View

Am useing 1:20 ratio (w/v) for hydrolysis of corncob powder and getting 10gL xylose.,how to calculate percentage acid hydrolysis in biomass?

kindly help me to calculate percentage acid hydrolysis of corncobs with sulphuric acid (1%), am getting 10gL Xylose during process

06 July 2016 9,985 3 View

Why RID in HPLC is ideal for the quantitative estimation of sugars ? Why not PDA ?

Why RID in HPLC is ideal for the quantitative estimation of sugars ? why not PDA ?

05 June 2015 6,418 4 View

Does anyone know of a method for spectrophotometric estimation of hydroxy methyl furfural?

02 March 2015 4,571 1 View

What's the difference between aldoketo reductase and aldo-keto reductase?

I am looking for phylogenetic analysis is aldoketo reductase family protein, while searching the sequence related to the same in NCBI. Aldo-keto reductase and aldoketo reductase giving different...

01 February 2015 4,285 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View