I run the process without vacuum filter and vacuum dryer. But could not extract the film. Can anyone help me or show me other way to formulate the film electrode
You can use Dip-Coating method or Drop-casting to make films.
I have used mosaic to new raster tool to join two aerial image from Landsat 8 of different path. However, raster files had color variation and after mosaic I got a straight line running from...
22 December 2020 4,640 4 View
How to define population proportion (prevalence) for sample size determination? We often see the equation as: Sample Size= Z2.p.(1-p)/d2 in this particular sample size determination equation which...
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I have been studying and working on research methodology for at least five to six years but still I could not satisfy my self with the answers and references provided in the books and web...
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Hi All, Recently, I purified 8 proteins and stored at -20 with the elution buffer containing 20 mM Tris, 250 mM NaCl and 200 mM Imidazole. I autoclaved the buffer, so I do not know whether the...
25 September 2020 9,814 6 View
Hi All, We recently used HisPur-NiNTA resin for protein purification. The protocol from Thermo says that 20 mM MES,100 mM NaCl, pH 5 is required to regenerate beads without affecting Ni content....
14 August 2020 4,083 3 View
Hi What are the best simulators/emulators that provides a wide support to simulate attacks on iot devices and protocols (BLE, MQTT, authentication, MITM, ddos, sybil ...) and that offers by the...
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31 August 2019 5,806 3 View
Hi all, I am planning to PCR a 7430 bp fragment from a big plasmid(11 kb) with Thermo Phusion hot start II DNA pol. Thermo recommend to use 15s-30s as extension time per 1 kb length. Overall GC%...
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Hello All, I just want to know your thoughts on NEB Q5 site directed Mutagenesis kit. Has anyone used this ? Is it good ? I have a plasmid of 6 kb on which I design my SDM . Your advice and...
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21 May 2019 7,011 3 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...
03 March 2021 7,975 3 View
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03 March 2021 6,317 1 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...
01 March 2021 6,722 1 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View