What is the role of calcium in cell life?

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What's agile diagram ?

details of agile diagrams in a flow chart

12 June 2024 4,430 1 View

Where do i get dataset in fuzzy set?

cCurrently, I am doing research in approximation of fuzzy set, I am looking for a real-world data set that is in fuzzy set to use.

22 May 2024 6,520 2 View

MDR1 (ABCB1) western blotting?

Has anyone seen MDR1 bands below 75 kDa? I am using a monoclonal antibody and I am expecting a band at 170 kDa

15 May 2024 8,690 4 View

How can I install TRNSYS 18?

Hi there, I am looking for ways to install TRNSYS 18 free

27 April 2024 583 1 View

Can I obtain a sample of the MAACL-R form by Marvin Zuckerman?

It is a form of 132 adjectives used to measure affect, such as Anxiety, Depression, Hostility, and Positive Affect.

22 April 2024 6,628 0 View

Why can't i download my article to my page?

Use of a maltodextrin-based feed with a lysozyme product to ... Kindly add this article to my profile. Thank you very much Regina

16 April 2024 9,905 0 View

How can I dissolve my drug in 0.5% methylcellulose?

I am preparing a drug for oral gavage which needs to be dissolved in 0.5% methylcellulose. The drug powder remains as clump and refuses to go into solution. Any tips on this?

01 April 2024 4,859 3 View

How do I revise the title of an article I on Reserach Gate?

Hi, My recent publication "Exploring cross-boundary collaboration for youth mental health in Sweden – a qualitative study using the integrative framework for collaborative governance". Is wrongly...

27 March 2024 3,153 2 View

What are the data required to assess the impact of climate change on agriculture and food security?

all relevant data required in conducting this research.

10 March 2024 7,026 3 View

Why do we predominantly utilize the Caputo sense of AB derivative over the Riemann sense in modeling real-life problems?

1. Additionally, what do we mean by the terms "memory preserving" and "heredity property" in this context? 2. How can we determine the appropriate fractional derivative, or kernel, for modeling...

04 March 2024 4,843 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View