Experiment or Quasi Experiment Design?

What kind of study design are we talking about? Subjects are randomly assigned to one of three groups. In addition, a moderator and a mediator are collected as continuous variables. Is this an...

15 January 2021 1,809 4 View

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

What is the best solvent for the extraction of chemical compounds that have an insecticidal effect ?

26 November 2020 4,609 15 View

How can I manually add co-authors when uploading a published work?

How can I manually add co-authors when uploading a published work?

14 October 2020 721 3 View

How do i compare differences in rankings?

I am writing a dissertation on the effects of a new accounting standard on financial statements. I have calculated several ratios for before and after the implementation of the new standard. I...

23 August 2020 4,019 5 View

Nvivo - how to extract and analyze hashtag?

Hello, I am using Nivo 12.0 plus. I've a dataset of tweets, unfortunately not extracted by NCapture. How can I extract and analyse hashtag? Is it possibile using auto-code functions? Thank you

19 August 2020 9,532 3 View

What is a scoping review?

In what context should we choose to do a scoping review? How is it different from systematic review, literature review, mapping review and Cochrane review? What are its mandatory or optional...

05 July 2020 8,206 3 View

Does safety always increase by increasing system reliability ?

System Safety and Reliability are fundamental to system design and involve a quantitative assessment prior to system development. An accurate prediction of reliability and system safety in a new...

31 March 2020 8,969 14 View

If I use REVEL score as a meta-predictor for clinical variant classification, what would be a reasonable cut-off to filter disease variants?

Ioannidis et al., 2019, reported the development of the REVEL score (rare exome variant ensemble learner) for redefining pathogenic variant classification, and Tian et al., 2019, reported that...

29 March 2020 2,450 2 View

Is there a method to statistically compare two or more regression coefficients in paired data?

Hello, I have conducted simple linear regression for four time points, always on the same sample. My time points are 10 years apart and now I wish to compare the slopes / betas / regression...

21 March 2020 10,020 9 View

Is the Case Study Method eligible to create theory?

I have been conducting research for PhD Theses using case study as a method, mainly refer to Robert K. Yin works. At the final step of my research, I define some concepts and use the concepts to...

12 February 2020 3,146 10 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View