What is the role of ascorbic acid when added during the labeling by direct method
I think it increase the stability after labeling process
You can see the following link
https://pubchem.ncbi.nlm.nih.gov/compound/ascorbic_acid#section=Drug-Labels-for-Ingredients
Could you please give specifics of your labeling protocol.
Dear, Dr. Safa Challan
It is act as strong stabilizing agent when being added to a such labeling reaction. Thanks for this scientific valuable question and have a good luck.
Best, regard
NASEER ALMUKHTAR
Dear safa
Ascorbate was an antioxidant that when added it to the reaction will be neutralise it to be more stable deu to scavenger activity of Vit.C...
I think all above answers around my answer
What is the method of assessment protein binding with radiolabeled compound in mice serum?
31 December 2019 4,456 4 View
What is the injected dose of TCA trichloro acetic acid for mice toxcicity intraprotenial?
11 December 2019 1,920 1 View
I think it's must contain an electrons donating group to be suitable for labeling by technetium 99m or iodine 125.
11 December 2018 5,125 2 View
The best method
01 February 2017 3,940 4 View
fundamental of nuclear pharmacy
01 February 2017 1,466 5 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...
14 February 2021 6,049 3 View
Dear all I have a 2-dimensional mode shape of one concrete beam and I put the data of mode shape in a matrix (69*3) which the first column in the matrix is x coordination of my beam and the second...
10 February 2021 3,301 3 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
09 February 2021 5,155 5 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...
01 February 2021 780 3 View