I have found increased acetylcholinesterase (AChE) activity in rat plasma after administration of Black seed oil. I have also found decreased AChE activity in rat plasma after administration of thymoquinone, an active component of Black seed oil. What does these results imply?
Thymoquinone(TQ ) is a major constituents of Black seed fixed and essential oil, which has been reported to restore oxidative balance, mitochondrial dysfunction, and cholinesterase activity caused by A β administration. TQ is usually present in the form of glycosidically bound aglycones, which easily cross the blood-brain barrier,.
Maybe because the other chemicals present in the black seed oil had affected the inhibitory activity of Thymoquinone. On the other hand, when you administered the isolated and pure Thymoquinone (based from your question, I am assuming that you administered Thymoquinone only and no other chemicals were included) the inhibitory activity became prominent. This implies that Thymoquinone is maybe an acetylcholinesterase inhibitor. Based from this article, quinones are AChE inhibitors. you can check this article
Measuring plasma level of AChE is not very reliable and you should assay it in brain or other tissues according to the aim of your study.
Acetylcholinesterase (AChE) acts primarily as a regulatory enzyme of cholinergic neurotransmission by hydrolysing Acetylcholine to choline and acetate. Therefore, inhibition of AChE enhances the ACh concentration there by restoration of brain cholinergic activity provides a possible symptomatic treatment option for Alzheimer's diseases.
Acetylcholinesterase (AChE; 3.1.1.7), plays a critical role in controlling the effects of Achetylcholine (ACh). AChE, hydrolyzes the neurotransmitter, ACh at the synaptic cleft of the cholinergic synapse and neuromuscular junctions. In case of AChE, AChE is expressed in a variety of tissues including nerve, muscle, white and grey matter of barin, placenta, hematopoietic cells and amniotic fluid. In additions of the AChE activity, AChE provides a potential target for the treatment of Alzheimer's diseases.
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