I am working on Bacillus cereus and Bacillus altitudinis. Currently, I am trying to induce the spore production of both sp. My initial setup was 30°C, 72 hours, and 150 rpm. B. cereus produced a significant amount of spores but B. altitudinis produced less than 10 colonies only. Then, I changed the temperature to 37°C (72 hours and 150 rpm). Yet, no spore from B. altitudinis.
This is the methodology I used:
- 5% of bacterial suspension in Luria Bertani media (liquid)
- Incubated at 37°C, 72 hours, and 150 rpm.
- Spore collection: Centrifuged at 6000rpm for 5 min and resuspended the pellet in phosphate saline solution (pH 7.0)
- Heat killed at 80°C for 20 min
- Cooled in ice
- Serial dilution and spread plating
- Incubate overnight at 37°C and CFU recorded
I am looking for an optimum condition to induce spore production in B. altitudinis. Please advise me. Thank you in advance.
Thank you for bringing these articles to my attention- I will enjoy reading them!
Try downloading this article from this link https://link.springer.com/article/10.1007/s11104-017-3195-z
Many factors determine the resistance properties of a Bacillus spore to heat, chemical and physical processing, including thick proteinaceous coats, peptidoglycan cortex and low water content, high levels of dipicolinic acid (DPA), and divalent cations in the spore core. Recently, attention has been focused on non-thermal inactivation methods based on high pressure, ultrasonic, high voltage electric fields and cold plasmas for inactivating Bacillus spores associated with deterioration in quality and safety. The important chemical sporicides are glutaraldehyde, chorine-releasing agents, peroxygens, and ethylene oxide
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