How to neutralise a peptide in a peptide-protein interaction?

Hi all, I am performing an IP using 20AA biotinylated peptides as bait and recovering peptide-protein complexes using streptavidin beads. I elute the proteins using heat, SDS and mercaptoethanol,...

15 December 2019 568 2 View

What are the basis of selecting a variety based on physiological WUE?

My question is which genotype/variety is best? The one with higher WUE (Pn/gs)? If so, then high WUE is associated with a lower yield. Is it means we are looking for the genotype which is best at...

09 December 2019 4,608 1 View

How can i distinguish between organic and inorganic material ?

i want to separate organic material and inorganic material.

10 January 2018 6,139 3 View

Which antibody is better DO-7 or DO-1 for p53 (tumor suppressor gene)?

I read some papers and most of them listed DO-7. Also, what other antibodies are better for p53 detection in terms of quality and reactivity and that are cost effective.

10 July 2017 9,185 6 View

Why many researchers use bead on plate weld type and not butt joint weld?

Various journal papers show that many researchers study the effect of process parameters by using bead on weld type and not butt joint weld.e.g. almost all papers on activated tig welding show...

18 April 2017 3,526 12 View

Drug treatments affect B-actin?

Hi I recently performed some western blotting and have noticed that my treatment has reduced B-actin expression in cells.Do certain drugs affect B-actin expression? This result has been consistent...

13 February 2016 5,819 3 View

What marker to use as a control for western blotting in hypoxia?

Hi, I wish to perform western blot analysis to detect some proteins under hypoxia and normoxia. I cannot use b-actin as my control marker as these levels tend to change under hypoxia. Can anyone...

31 January 2016 674 5 View

How to select parameters for RSC-ZCS Medium Voltage High Power step up DC-DC converter for offshore wind application?

I am doing simulation of Resonant Switched Capacitor (RSC) step up converter with zero current switching (ZCS) in MATLAB. But I am not able to get exact output that I want. and I think reason is...

06 January 2016 3,920 3 View

Can EDAX charecterization be used to find percentage of nano particle added in epoxy resin?

25 December 2015 2,583 8 View

In an energy harvesting wireless sensor network, what is the typical amount of energy required to activate the energy harvesting circuitry?

Are there any research papers that consider the energy required to activate the energy harvesting circuitry?

16 December 2015 4,754 2 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View