Excitation spectra vs Absorbance spectra?

I have excitation spectrum at 370 nm of wavelength when 520 nm of emission wavelength. But I did not get any of absorbance at 370 nm in UV-Vis spectrum for same sample. What is the reason for...

06 July 2018 4,970 0 View

How to prevent TEM grid from damage while exposure with electron beam at high magnification?

10 November 2016 451 10 View

How to convert potential from Normal Calomel Electrode to Reversible Hydrogen Electrode?

The pH is 12, Thank you in advance...

01 February 2016 8,182 1 View

How can I find particle size for fluorescent nano metal clusters or quantum dots using dynamic light scattering particle size analyser?

I am getting always wrong size of particles (size calculated from HRTEM image, and UV-Vis spectra) for fluorescent gold clusters and carbon quantum dots. but DLS spectra gives reliable results for...

01 January 1970 7,806 1 View

Aggregation Induced Emission (AIE) and Aggregation Induced Emission Enhancement (AIEE)

I want to know the mechanism of electronic state & its changes during Aggregation Induced Emission (AIE) and Aggregation Induced Emission Enhancement (AIEE). Some reports says the reason for...

01 January 1970 4,990 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View