What is the reagent that makes the lysis of blood cells, which also improves the yield of DNA recovered ?

Liliane Tavares de Faria Cavalcante @Liliane-Cavalcante

25 August 2016 1 3K Report

I am looking for a reagent that makes the lysis of blood cells in order to improve the yield of DNA recovered by the use of total DNA extraction kit. Does anybody know this reagent ?

Sibnarayan Datta

Hi Dr Faria,

You can use RBC lysis buffer for specific lysis of the RBC present in the blood (human), followed by centrifugation of the WBCs , resuspension in lysis buffer and DNA extraction.

You may refer to the methods section of the attached paper.

Best wishes,

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