IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to delete a copy of a paper, and how to delete a co-author's name?

I mistakenly uploaded a copy of the same paper and entered my alternative name. How to delete them out? (I couldn't navigate to the right buttons to do it. So from where to where is what I don't...

07 January 2021 7,694 6 View

How can Meta-Analysis be applied to the Life cycle assessment (LCA) of Wastewater treatment Plants (WWTPs)?

I am new into the meta-analysis concept. Having done a little reading and watched videos on YouTube, I understand there are different kinds of meta-analysis. And the type of summary statistics or...

29 December 2020 3,677 3 View

Best Way to Preserve Nitrifying Bacteria in Liquid Culture?

I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...

18 December 2020 5,411 4 View

HPLC data show negative peak on the blanks, please can I solve the problem?

09 November 2020 5,884 5 View

Any molecular beacon producing company to recommend ?

Hello, For one of my projects I would like to test a Live RNA imaging using molecular beacons such as in this paper : Molecular Beacon-based Detection and Isolation of Working-ty... As we...

28 October 2020 9,457 2 View

MsaClustalW() Take ages. What to do ?

Hello, I'm new to the bioinformatics world and would like a feedback on which MSA tool to use. I want to find a consensus sequence in about 18000 sequences (from marine biology samples) from...

19 October 2020 2,607 2 View

What is the best way to store PBMC after isolation?

So I need to store these for 4 weeks at least, before I do a Dyna bead negative T cell isolation on it. What should I store it in FBS? or DMSO and at 4 degrees? or is -20 not good for the cells?

26 July 2020 2,339 3 View

Best way to preserve non-sporulating bacteria?

What is the best way to preserve non-sporulating bacteria for further study?

20 May 2020 5,532 5 View

Best way to preserve heterogeneous culture of non-sporulating bacteria?

I have a culture of heterogeneous bacteria that will NOT form endospores. This bacteria was originally isolated in nature and is a complex culture of multiple species. I want to be able to...

20 May 2020 8,369 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View