Hello everyone,
we are using High Capacity NeutrAvidin™ Agarose beads to separate biotinylated proteins. For screening experiments we'd like to reuse the beads. Therefore, I am wondering if anyone has experience with the reuse of Neutravidin (or Streptavidin) Agarose resin beads and possible a protocol he or she is willing to share.
Thanks in advance!
HI,
It is only possible if you are using a biotin variant, such as iminobiotin, which displays pH sensitive interaction with avidin. However, if you are stuck with normal biotin, atleast I have not been able to find an elution buffer that would elute biotinylated proteins (which itself is a challenge) without damaging beads.
Good luck, and if you find an elution buffer that helps, please share.
Neha
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