How can i collect Bursa of fabricius from Japanese quail without damage to it.
Same procedure as for removal of bursa in chicks but with a little more care because of smaller size of quail chick.
The bursa of Fabricius is an epithelial and lymphoid organ develops as a dorsal diverticulum of the proctadeal region of the cloaca means. It is located right on the dorsal side of proctodeum in birds. It is easy to identify because of the presence of as many as 15 primary and 7 secondary plicae or folds. However, quail bursa is smaller than chicken so not easy to locate.
I order to remove bursa, sacrifice the bird, and cut open the abdominal cavity to locate large intestine specifically where caecas are attached. The bursa will be located down to this region. Carefully remove large intestine from stomach wall, find a globular gland present on the dorsal side just before cloacae, which is bursa. To remove undamaged bursa, make an extra cut from both the side, and then trim it to get only bursa.
RP Singh wrote detail of procedure, you can put your finger under the tissue and gently retract it up, to make make it prominent. Then cut it with scalpel or scissor to remove from the body.
Position and size of bursa in a day-old chicks is attached herewith for guidance.
Others above have properly described the surgical methods to remove the bursa. However, why are you collecting the bursae? Is it for study purposes or is it removing them to have clean bird for eating?
very nice explanations and illustration by Singh and Ahmed. the procedure is the same as for chicken, but with much more cares, due to the smaller size.
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