How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

I teach a STEM career course for IT majors at a small liberal arts school. I am looking for collaborators/information sources for a new book ?

Looking for collaborators/info sources for a new book I am writing in the career/life coaching field on "Adult Bullies". Most research has been done on childhood bullying, however they eventually...

27 November 2020 6,532 1 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

Is annealing should be done again after a year of nano-particles fabrication and they are found to be agglomerated?

I have prepared CeO2 based nano-particles. due to some technical issues some of the properties like electronic structure and magnetic properties are pending. the samples were prepared...

08 October 2020 769 3 View

Advice writing an unbiased qualitative research question?

Hi, I was wondering whether kindly souls out there might be able to help me with some advice regarding the formulation of a RQ for my qualitative study please? I'm trying to refine an early...

25 March 2020 6,512 5 View

Is there any python code which converts text(unstandarized) into a csv fom?

I have an unstandarized text data . So when I convert the text into csv form it results are not into a proper csv format.

05 February 2020 4,556 3 View

Do you think the word/phrase "Economic Growth" is an innocent and positive one?

15 January 2020 2,890 14 View

What ML/AI algorithms can I use to analyze Bank statement and detect frauds??

Ml algorithms which can be useful to analyze bank statement and then extract information about that frauds.

08 January 2020 9,890 6 View

Any free software to convert my audio interview to text ?

I am doing Qualitative Research

31 December 2018 4,092 8 View

What are barnacles environmental constraints ?

I've got some data on barnacles across a shore line and was after a guide to the appropriate review that discuses the biochemical changes within barnacles during desiccation. Is there one that...

03 December 2018 7,280 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

Can you please suggest me how to convert EDF file into Mat file for EEG datasets?

Hi sir, 1.can you please suggest to me how to convert the EDF (European Data format) file into a Mat(Matlab) file for EEG datasets?. 2. Also suggest some publicly available EEG Motor Imagery...

15 February 2021 7,620 1 View

Is Genetic Algorithm usage for Motor Conception FEA optimization a good choice?

Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...

14 February 2021 1,160 7 View

How to interface ANSYS Maxwell and Matlab?

Dear All, I am working on electric motor design in Ansys Maxwell. I have written optimization code in MATLAB. I want to run this code in ANSYS MAXWELL and generate FEA results. Does anyone know...

10 February 2021 1,279 1 View