procedure for isolation of rhizoshere phosphate solublizing bacteria
you can go for selective isolation on Pikovskaya's broth and streak isolation on pikovskaya''s agar
You try this media :Sperberg’s hydroxy apetite medium (Sperber, 1958). Serial diltuion and plating on these media and incubateat 30°C for 48 h. Observe the diameter of the clearing zones around the colonies
which character are similar between Gram positive bacteria and mycoplasma
11 December 2015 1,056 2 View
shelf life in terms of its stability, in case if temperature, pH etc
07 August 2015 5,514 2 View
generally from plant poweder through extraction in vitro antiidant activity we are measured but in vivo in plant how induce stress and which parameters were measure?
07 August 2015 9,463 1 View
Role of women especially in agriculture in India as well as other county.
07 August 2015 9,312 4 View
for plant growth required N, P, K (macro), micro element so, which form will absorb by plant root
07 August 2015 2,086 2 View
which bacillus spp. cause disease in human?
06 July 2015 5,477 0 View
Generally plant drug is dissolved in DMSO for antimicrobial activity. so is it toxic?
06 July 2015 7,273 5 View
Rope of humus in agriculture
05 June 2015 8,176 1 View
what is the mechanism of superoxide anion free radical scavenging assay of plant extract?
04 May 2015 2,818 5 View
list of bacteria which causes diseases in cattle friquently
03 April 2015 9,767 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View