What is the procedure for isolation of rhizoshere phosphate solublizing bacteria?

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Which character are similar between Gram positive bacteria and mycoplasma?

which character are similar between Gram positive bacteria and mycoplasma

11 December 2015 927 2 View

How increase shelf life of plant extract?

shelf life in terms of its stability, in case if temperature, pH etc

07 August 2015 5,369 2 View

How measured the in vivo antioxidant in plant ?

generally from plant poweder through extraction in vitro antiidant activity we are measured but in vivo in plant how induce stress and which parameters were measure?

07 August 2015 9,324 1 View

What is the role (contribution) of women in agriculture?

Role of women especially in agriculture in India as well as other county.

07 August 2015 9,206 4 View

Plant absorb nutrient in which form?

for plant growth required N, P, K (macro), micro element so, which form will absorb by plant root

07 August 2015 1,913 2 View

Which bacillus spp. cause disease in human?

which bacillus spp. cause disease in human?

06 July 2015 5,250 0 View

What concentration of DMSO is toxic for cells?

Generally plant drug is dissolved in DMSO for antimicrobial activity. so is it toxic?

06 July 2015 7,102 5 View

What is the humus?

Rope of humus in agriculture

05 June 2015 8,046 1 View

What is the mechanism of superoxide anion free radical scavenging assay?

what is the mechanism of superoxide anion free radical scavenging assay of plant extract?

04 May 2015 2,676 5 View

Which bacteria cause disease in cattle?

list of bacteria which causes diseases in cattle friquently

03 April 2015 9,535 0 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Phosphate buffer with different pH conditions: HCl or NaH2PO4?

Hi all, I just encountered this dogma; when adjusting pH of phosphate buffers, I've been using HCl/NaOH to in/decrease pH of buffers and recently I was told that HCl/NaOH can't be used to adjust...

08 February 2021 9,070 5 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View