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The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?
02 March 2021 4,511 3 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...
23 February 2021 6,266 3 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
Which variables (such as pull speed, melt temperature, annealing temperature/duration, cooling rate, etc.) affect the grain size of czochralski-grown polycrystalline silicon? In what direction...
10 January 2021 2,204 6 View
Good day. I am doing a studu on the use of fish scales to create a nano filter. This is aimed at potentially solving the face mask problem brought about the pandemic. This is what I know so...
09 January 2021 8,907 5 View
How would you calculate inter-coder reliability (Kappa coefficient?) for the following scenario: 6 texts coded across 7 categories (codes) by 3 coders?
23 December 2020 2,324 3 View
Dear all, I recently bought a RTX3080 and was unable to start the NPT and production steps due to lincs warning (my old gtx 1080ti card ran normally, using the same .mpd), I tried several...
18 December 2020 3,333 2 View
It is possible to notice, at some three phase induction machine datasheets, that exists a relationship between stator leakeage reactance and slip, that is, the stator leakage reactance increases...
23 November 2020 2,949 3 View
Hello everyone, I am having troubles with the bubble point measurement to find the pore size of my self-fabricated microfiltration membrane, and I couldn't find a proper answer anywhere else. It...
16 November 2020 7,574 1 View
Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...
21 February 2021 374 5 View
Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...
10 February 2021 3,392 4 View
I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...
04 February 2021 503 3 View
I used Cochran (1963) sample size formula, but as it shows it so old and my advisor asks for some new references for finding an appropriate sample size.
01 February 2021 8,275 4 View
I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance
11 January 2021 3,346 2 View
How does one combine the basis of Quantum Physics that the information cannot be destroyed with the GR statement that black holes destroy the info?
14 December 2020 7,845 19 View
My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.
06 December 2020 7,964 6 View
Both have their own benefits and constraints too but in overall aspects which will be recommendable to farmer for sustainable agriculture.
23 November 2020 6,313 42 View
I am currently researching a network of species movement between temporary ponds using Least Cost Paths. With Conefor I have calculated the PCnum, EC(PC), and PC (probability of Connectivity, as...
23 November 2020 6,298 3 View
Mulching in agronomic crops is also mentioned in many literature
22 November 2020 631 48 View