I'd like to administer Rhodamine B-AgSiO2 nanoparticles that I commercially purchased to mouse brain via carotid artery. The solution I have has the concentration of 0,25 mg/ml. What should be the optimum concentration or can anybody suggest a range? I saw 50-100 ug/ml in papers regarding cell culture. And when shall I do the live imaging?
Steingrimur Stefansson
Hi Zeynep, rhodamine is a relatively stable fluorophore that doesn't bleach easily so I wouldn't worry about that.
What is a concern is that Ag forms a very stable complex with -SH. Serum albumin and glutathione are abundant in blood and will form complexes with Ag NPs.
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