What is the optimual condition for NHS/EDC reaction?

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Marie Berthuel

Hello Laleh Ghasemi ,

Usually, I use MES buffer at pH=6 with a concentration of 50 mM in distilled water. The ratio of EDC NHS is 1:1 and the concentration would depend on your experimental conditions and reactants.

Best regards,

Marie B.

Laleh Ghasemi

Dear Marie

Many thanks for your reply and help. It was really helpful to me.

Warm regards

Laleh

Houman Kholafazad Kordasht

I agree with Marie Berthuel