How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Is there any pipeline for 16s metagenome bacterial classification based on their functions?

In 16s rDNA metagenome analysis, after sequencing data. I want to classify bacteria based on their functions. I am looking for a Pipeline or any software that can do this job?

13 January 2021 533 6 View

What regulatory documents, international standards and forums have information about hot taps (hot-tapping) and how to use them safely?

My master's thesis deals with the use of hot-tapping in pipeline repair. For my master's graduation work I really need references to regulatory documents and various international standards...

10 January 2021 6,815 3 View

What is biquad filter?How pipelining retiming technique is done on biquad filter?

How to do pipelining retiming on biquad filters?

10 January 2021 7,209 1 View

How to calculate the flow rate required for fluidization for a fluidized bed gasifier ?

We need to calculate the flow rate of air required for fluidization of the bed. We have a compressor which gives air at 7 bar and a 1 bar rotameter for measuring the flow rate in LPM. How can I...

16 December 2020 2,092 3 View

Any threats faced by the pipeline industry?

For example if we say urbanisation is one of the threats. How can urbanisation affect the pipeline network?

06 December 2020 7,570 2 View

How to quantify lncRNA/miRNA in ribosomal depleted RNA-seq?

I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...

11 November 2020 5,331 1 View

Variation of image intensity with changing frame rate in G-stream pipeline?

Hello All! I'm a beginner to the Gstream pipeline. Please help me in understanding the importance of frame-rate in Capsfilter in this pipeline. In my current project, the pipeline is given as...

01 November 2020 4,798 1 View

If one wants to link together power sources all across North America, would it be better to use DC transmission lines, or gas pipelines, say with H2?

Intermittent renewable energy sources become more efficient and reliable as the number and diversity of sources is increased. But electrical transmission over distances greater than 2000 miles...

20 October 2020 731 5 View

What are the best pipleline available for the RNA-Seq data analysis ?

What is the latest pipeline to run the RNA-Seq genomic data ?

12 October 2020 7,618 2 View