When I measured the concentration of p53 for healthy people and patients with breast cancer, I found that the concentrations were very close and this is contrary to the belief that patients with breast cancer have a high concentration of this protein. I would like any researcher in this field to tell me about the normal conc. of p53 for healthy and patients ..... thank you
You would have to check the mutational status of your p53. Even if levels are high if it's mutated and cannot bind DNA it will have no suppressive effect effect
People with breast cancer suppose have low p53 gene expression or defective p53 protein. I don't understand why u said patients with breast cancer supposingly will have higher concentration in p53?
as I know, P53 is tumor suppression gene and it will arrest the cell cycle if it goes wrong
You would have to check the mutational status of your p53. Even if levels are high if it's mutated and cannot bind DNA it will have no suppressive effect effect
According to our IHC research of p53 in human prostatic carcinoma, this protein is highly elevated in cancerous prostatic glands (with higher Gleason pattern), but not in other surrounding-healthy tissue. Because we did not evaluate the mutational status of the p53, as A I Papadakis proposed, we are not sure of whether this p53 is elevated because it is unfunctional and thus feed back loop for expression is overstumulated, or it is functional and overexpressed due to its normal function say to kill cancerous cells in apoptosis.
The results of Miha may be true. Wild type P53 has a half life of only 20 minutes. However, under various stressful condition including oxidative stress/radiation etc that damages/mutated DNA, the degradation of p53 stopped and there is substantial induction of p53. Since, cancer cells faces several types of stresses, so they nay have high level of p53. However, since these p53 are mutated they are non-functional. So, I did not get the question of Mavis Lim? Why the cancer cells are supposed to have low level of p53????
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research