During the ultrasonic welding with PP, I observed somes bubbles near to the energy director. I would like know the nature, or the composition of them.
Greetings, Recently, I performed a Cycloheximide chase assay to assess the stability of transiently transfected proteins in HEK293 cells. The CHX chase assay was conducted for 12 hours with...
18 May 2022 8,818 1 View
Hi all! I'm a young researcher (doing my master's) and I'm trying to get a hold of AUCs in ROC curves for my research protocol. I'll be using a software, but i'd like to understand...
15 September 2021 6,574 1 View
I would like to phenotype M1 and M2 microglia in some specific brain region of the mouse brain. I am currently looking specifically at antibodies against Arg-1, iNOS and CD206... Could anyone...
09 March 2021 8,776 2 View
Hello ! I would like to extract gDNA for soil to do NGS. We have the QIAGEN Powersoil kit. I did it twice now. First, according the kit instruction : concentration = 10 ng/uL, 260/280 = 1.4,...
21 October 2020 1,233 4 View
Hello everyone, My name is Elodie and I'm PhD student in biochemistry and molecular biology. I'm trying to use TSA_CRAFT software which is a free software to analyse thermal shift assays datas...
23 June 2020 3,725 0 View
Hi, I am trying to use predefined field from output database on a simple example : a spring in a cylinder. First I did a calculation without predefined field. Second I used this result as...
28 April 2020 6,715 0 View
Hello folks, I know that it is possible to determine the chemical structure of a polymer by mass spectrometry even without sample preparation (using a DESI source or a solid probe APCI source...
05 March 2020 2,471 3 View
Hi guys, I would like to evaluate myeloid (monocyte/macrophage/Dc) content of my tissue lysates by q-PCR. I did it the same with T cells by quantifying rearranged TCR DNA which is specific to the...
29 July 2019 7,122 0 View
In my research, I aim to explore the use of languages in the bilingual classroom. As educators must arranged differently the two languages in the curriculum, it is important to ask, How do...
22 March 2019 6,173 4 View
Hello everyone, We are looking to buy a new GC-MS in our lab and we are wondering if chemical ionization (CI) can bring us some useful extra information. In my lab, we investigate unknown...
25 July 2018 6,734 8 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
29 July 2024 7,714 4 View
In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View
Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...
28 July 2024 5,771 2 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
I am looking to purchase a photography kit for onsite metallurgical investigations, such as capturing photos of worn areas, hot tears, cold tears etc. Could anyone suggest me a good...
22 July 2024 3,490 2 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
I want to reduce simulation time in CST for frequency domain solver by upgrading my computer. I already have discrete GPU but it doesn't seem to make any difference at all compared to integreted...
10 July 2024 2,789 4 View